Fig 1: Reduced content of UQCR and Atp5 measured by IHC in LBW kidney. Immunohistochemistry (IHC) analyses of NBW and LBW kidney sections obtained from 4-week-old rats. UQCR7 was expressed in glomerular tufts (blue arrows), in arterioles (mainly by vascular smooth muscle cells) (yellow arrows), and along the apical membrane of distal tubules (red arrows) (a–c). On the other hand, UQCR expression was markedly decreased in LBW rats (e–g). Atp5I was positive in tubular cells in NBW rats (d). Atp5I expression was weakened in LBW rats (h). The numbers of UQCR7-positive tufts per glomerulus (i), semiquantitative scoring of the UQCR7 staining intensity in the glomerular vascular pole (j), the rates of UQCR-positive cells in distal tubular cells on their apical membrane (k), and the percentages of Atp5I-positive tubular cells (l) were statistically analyzed. * p < 0.05 by unpaired t-test. ** p < 0.01 by unpaired t-test.